Hello. This is just a short report done individually on enzyme. Write two to three pages should suffice including tables/graphs. The report must include.
1. Title
2. Introduction
Describe what amylase does. What is the substrate? What is the product? What parameter are you going to examine (pH, salinity, temperature)? How do you intend to follow activity (Lugol’s Iodine approach)?
3.Methods
You do not have to write out the method of obtaining and preparing the salivary amylase or describing the Lugol’s Iodine assay approach. Just say that you followed the instructions in the lab manual. You do have to describe the pH values, temperatures or salinity concentrations you are using. Mention what negative/positive controls you set up, if any.
4.Results
Tabulate your data. I realize that the results are not quantitative e.g., dark, lighter, etc. Make sure you insert photos of the plates you were using and include a figure legend (descriiption) under the photo(s).
5.Conclusions
Discuss your data. Did you get a clear optimum condition for enzyme activity? Why do you think there is an optimum condition of pH or temperature? How would you improve the experiment? Perhaps with a modified range of temperature and pH values? I have to admit that the salinity experiment was one I had never seen before, and I am interested in hearing about the results again. You might want to look up online if somebody has done this experiment and what they got which may help you to make comments. Anyway, just speculate what may be going on at least. That is always welcome.
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