1. Include a photo of your western blot. 2. Graph the log10 of the molecular w

1. Include a photo of your western blot.
2. Graph the log10 of the molecular weight of the ladder proteins on the y-axis, and the Rf’s on the x-axis.
3. Get an equation for the line, with R^2.
Your BSA lanes might have one, two, or three bands.
If you have one band, it is BSA.
If you have two bands, one is BSA and one is a BSA aggregate (some BSA molecules can stick to each other to form one larger molecule)
If you have three bands, the thin band at the top could be an even larger BSA aggregate, or possibly some BSA that somehow got stuck between the stacking gel and the resolving gel.
4. Use your equation to calculate the mass of the BSA and the mass of the one BSA aggregate.