Category: Biochemistry

You are asked to analyze the amino acid sequence of a peptide PEP of 12 amino acids. You decided to
carry out extensive tryptic cleavage on the peptide first. As a result, two smaller peptides were obtained
and studied individually. (A) For the first peptide (PEP-1), amino acid analysis showed the presence of
only two types of amino acid, valine and arginine. MALDI-TOF of PEP-1 showed a peak at m/z 670.
(B) For the second peptide (PEP-2), after collision induced dissociation (CID), it yielded a mass
spectrum with the first four y-type ions at the following m/z values: 132, 269, 416 and 544. Deduce the
partial sequence of the original peptide PEP.

please solve the following questions ( in case of a number *10^ a number please convert it to decimal)

please solve the following questions ( in case of a number *10^ a number please convert it to decimal)

Examine the 2 pairs of proteins below. In each case, these are enzymes that catalyze the
same reaction in different species. The question is: are they related or not? i.e. Are the
structures essentially the same? Often you can get similar reactions from proteins that
evolved from a common ancestor (e.g., trypsin and chymotrypsin). Sometimes the same
reaction can be catalyzed by proteins with very different evolutionary origins and with very
different structures (e.g., trypsin and subtilisin). Your job is to decide if these proteins have
fundamentally different folds or if they have basically the same fold. Briefly justify your
answer (≤5 sentences).
1. Aminopeptidases from a mammal (PR1A) and the malaria parasite (PR1B)
2. Bisphosphoglycerate mutase from a mammal (PR2A) and the toxoplasma parasite
(PR2B)

Examine the 2 pairs of proteins below. In each case, these are enzymes that catalyze the
same reaction in different species. The question is: are they related or not? i.e. Are the
structures essentially the same? Often you can get similar reactions from proteins that
evolved from a common ancestor (e.g., trypsin and chymotrypsin). Sometimes the same
reaction can be catalyzed by proteins with very different evolutionary origins and with very
different structures (e.g., trypsin and subtilisin). Your job is to decide if these proteins have
fundamentally different folds or if they have basically the same fold. Briefly justify your
answer (≤5 sentences).
1. Aminopeptidases from a mammal (PR1A) and the malaria parasite (PR1B)
2. Bisphosphoglycerate mutase from a mammal (PR2A) and the toxoplasma parasite
(PR2B)

Examine the 2 pairs of proteins below. In each case, these are enzymes that catalyze the
same reaction in different species. The question is: are they related or not? i.e. Are the
structures essentially the same? Often you can get similar reactions from proteins that
evolved from a common ancestor (e.g., trypsin and chymotrypsin). Sometimes the same
reaction can be catalyzed by proteins with very different evolutionary origins and with very
different structures (e.g., trypsin and subtilisin). Your job is to decide if these proteins have
fundamentally different folds or if they have basically the same fold. Briefly justify your
answer (≤5 sentences).
1. Aminopeptidases from a mammal (PR1A) and the malaria parasite (PR1B)
2. Bisphosphoglycerate mutase from a mammal (PR2A) and the toxoplasma parasite
(PR2B)

Learning Goal: I’m working on a biochemistry writing question and need the explanation and answer to help me learn.
I am taking recombinant DNA class and I need help in doing a CRISPR write up and will provide all the info later. I need to find sources related to my research question and analyze the articles and relate them to my research question and will provide all the info I need someone to be expert in that.

Pharmacists’ Patient Care Process (PPCP) Research Paper
The PPCP Research Paper should be 1‐2 pages, including references, and answer the following questions with peer‐reviewed references to support your answers.
**** Make an argument and choose three articles from (KIDNEY STONES) from the link that I uploaded that SUPPORTS your argument from.
and by that you will be answering those questions :
Questions to be answered:
1. What is the relevance of the patient’s ethnicity (genetics)?
2. What is the relevance of the patient’s diet (epigenetics)?
3. How do animal studies contribute to our understanding of the problem?
4. Have clinical research studies supported conclusions drawn from animal studies and is there a current clinical consensus regarding genetic factors, epigenetic
factors, and medical interventions? If not, what studies are needed to resolve any controversies?
5. What is the likely biochemistry underlying the development of the patient’s problem and resolution of the problem?
A minimum of three references are required:
1. One or more describing genetic factors that contribute to the patient’s problem.
2. One or more clinical studies that test possible dietary or pharmacological interventions.
3. One or more that define amino acids, other than glycine, that are likely to contribute to the development of the patient’s problem.
You can have discussions and share references with fellow students. However, the writeup should be written in your own words and NOT plagiarized.
The format for references should be: Author’s last name, Initials (publication year) Title of the paper, Journal name Volume (Issue): First page‐Last page.

Description
In this assignment, you will answer questions pertaining to material found within Module 4
Objectives
Discuss how the primary structure of nucleic acids is the order of bases in the polynucleotide sequence, and the secondary structure is the three-dimensional conformation of the backbone.
Discuss the monomers of nucleic acids are nucleotides.
Discuss how the bases are bonded to the sugars, forming nucleosides.
Summarize how nucleosides are linked by ester bonds to phosphoric acid to form the phosphodiester backbone.
Specific proteins called single-strand binding proteins bind to the single-stranded regions and protect them from nucleases.
Spontaneous mutation of bases and insertion of the wrong nucleotide would normally lead to an error every 104 to 105
Instructions:
Step 1: Read the article found in section 9A: Biochemical Connections: Law in your digital book.
Using the Concorde’s Online Library, your digital material, and other scholarly websites, discuss the following:
What are your feelings regarding the article?
Describe, in detail, what the following statement means, “What can be patented is purified DNA containing the sequence of the gene and techniques that allow the study of the genes.”
How do you see the patenting of genes/DNA impacting science and medicine over the next 30 years?
Do you believe this trend is ethical? Why/why not?
Step 2: Compile your thoughts & research and write a 3-5 page paper (without the cover page and reference pages), addressing the topics and questions above.
Step 3: Once complete, save your file and submit.
You are required to have at least two outside resources, at the scholarly level. Wikipedia is not acceptable. Neither are Encyclopedia Britannica, YouTube videos, dictionaries or any other reference books. You are encouraged to use
: GaleResourcesLinks to an external site.
Assignment Submission:
The use of outside resources is required and all papers must be cited and written in APA format.
Once you have completed the assignment, click the Submit Assignment option in the menu to the right and select a format for submitting your assignment.
Click the Submit Assignment button when you are ready.