1. Introduction – (1/3 – 1/2 a page) (include the # AND two letters of your Mixe

1. Introduction – (1/3 – 1/2 a page) (include the # AND two letters of your Mixed Unknowns at end of
Intro) = 6 pts.
2. Methods (Tests), Photos & following of Dichotomous Key = 10 pts. for each microbe = 20 pts.
total (see instructions for first unknown paper); refer to each microbe with a letter
Show photos of an isolation streak plate for your mixed culture (#), and for your 2 individual bacteria
3. Results & Conclusions (include clear documentation & labeling of photos) =10 pts. for each microbe
= 20 pts. total, including 4 points for EACH correct ID
**Please write about one unknown first (ex: Gram (+) followed by the other ex: Gram (-))
*Identify your microbe again by letter AND for the first time by Genus & species at the END of each
section.
4. Discussion of ONE microbe of your choice that was supplied to you on a posted list OR one of
the two microbes identified in this unknown project OR if your presentation is on a bacteria you
may discuss that (two (2) full pages) – Note: Your discussion must be at least two full pages = 27
pts. (15 pts. for content, 5 pts. for inclusion of references within text, & 7 pts. for length of two full
pages)
*You must have a minimum of three references in the body of your text (NOT Wikipedia or MicrobeWiki,
and NOT including your lab manual, although you may use your textbook as one of your references).
Please see instructions for the first unknown paper on HOW to reference and avoid plagiarism!
5. Conclusion (1/3 – 1/2 a page) = 6 pts. – *Do not repeat result. Please conclude the unknown
project.
6. Dichotomous Keys for both Gram (+) & Gram (-) organisms = flowcharts for BOTH Gram-pos. &
Gram-neg. microbes = 2.5 pts. each = 5 pts. total (Please staple to your paper or include photos
large enough to read.)
7. Highlight the names of your microbes along the route you followed on dichotomous keys for both
Gram (+) & Gram (-) microbe = 2 pts. each = 4 pts. total
8. Reference page = 6 pts. – References may be included on the same page with text, following your
conclusion. *Minimum of 3 references required.
9. Format & Grammar = 6 pts.
Double-spaced,12-point font (note: this is not 12-point font), < 1.5-inch margins. Grammar is important; please utilize the writing center if necessary. And the unknown bacteria that I found is "E. erogenes" Alphabetical List of Microbes (and the diseases they cause) that you may choose to write a 2-page discussion for your Mixed Unknown Report (Choose ONE only!) (Note: A “zoonotic” is a microbe that is transmitted to humans via animals.) o Agrobacterium tumefaciens (plant pathogen that causes crown gall disease in plants & has also been revolutionary in promotion of plant biotechnology) o Bartonella henselae (zoonotic that causes “cat scratch fever”) o Bordetella pertussis (pertussis/whooping cough) o Borrelia bergdorferi (Lyme disease) o Brucella melitensis (zoonotic that causes brucellosis/Malta fever/undulant fever) o Chlamydia trachomatis (STD chlamydia) o Clostridium botulinum (botulism/food poisoning/nervous system disorder flaccid paralysis & other) o Clostridium tetani (tetanus/nervous system disorder “lockjaw”/muscle rigidity & other) o Corynebacterium diphtheriae (diphtheria/suffocation) o Deinococcus radiodurans (not a pathogen - extremophile that is unusually resilient) o E. coli (gastroenteritis/UTI/other) and/or E. coli O157:H7 (hemorrhagic colitis/hemolytic uremic syndrome/other) o Francisella tularensis (zoonotic that causes tularemia; one of the most infectious bacteria known - ~10 cells are enough to cause disease!) o Haemophilus ducreyi (STD – “cancroid”/genital ulcers) o Haemophilus influenzae (pediatric meningitis) o Helicobacter pylori (peptic ulcers) o Legionella pneumophila (Legionnaires’ disease) o Listeria monocytogenes (listeriosis/often fatal food poisoning) o Magnetospirillum magneticum (not a pathogen - name should speak for itself!) o Mycobacterium tuberculosis (tuberculosis) o Myxococcus xanthus (“communicating bacteria” with unusual properties!) o Neisseria gonorrhoeae (STD - gonorrhea) o Neisseria meningitidis (bacterial meningitis) o Pseudomonas aeruginosa (opportunistic pathogen that infects primarily those with compromised immune systems, burn victims & cystic fibrosis patients - causes UTIs, respiratory infections, dermatitis, soft tissue infections, GI, bone & joint infections & systemic infections) o Rickettsia rickettsii (Rocky Mountain Spotted Fever) o Salmonella enterica (salmonellosis/food poisoning) o Salmonella enterica serotype Typhi (typhoid fever) o Shigella dysenteriae and/or Shigella flexneri (shigellosis/severe form of dysentery/diarrhea) o Staphylococcus aureus/MRSA (boils & impetigo (skin infections), food poisoning, cellulitis, osteomyelitis, pneumonia, endocarditis, staphylococcal toxic shock syndrome) o Streptococcus pyogenes (pharyngitis/”strep throat”, impetigo, erysipelas, scarlet fever, necrotizing fasciitis, myositis, endocarditis, streptococcal toxic shock syndrome) o Treponema pallidum pallidum (STD - syphilis) o Vibrio cholera (cholera/severe diarrhea) oYersinia pestis(bubonic plague)