Are there any challenges associated with the validation of liquid chromatography

Are there any challenges associated with the validation of liquid chromatography tandem mass spectrometry (LC-MS/MS) for voriconazole therapeutic drug monitoring (TDM) in urine samples?
Please write a research proposal regarding voriconazole therapeutic drug monitoring in urine sample by LC-MS/MS comparing to analysis this drug in serum samples which is the common used and applied method in most of the laboratories. Start with introduction then literature review, Relevance of the Research, Research Question, hypothesis, aims, methodology, and references.