In this scenario: You amplified and cloned the sequence for the first 10 AA of the gene homo sapiens ACE2 angiotensin converting enzyme 2 [NM_001389402 in genbank]. This sequence is inserted into the multiple cloning site (MCS) of the pDAWICKI (this is not a real plasmid) plasmid using the BamHI and EcoRI sites. The sequence of this MCS is listed below. The BamHI site is immediately upstream of your insert and the EcoRI site is immediately downstream of your insert. You want to study what this portion of the protein interacts with, so you decide to fuse the first 10 AA of your proteins to GST. The questions attached need to be answered.