write introduction and discussion (Electrophoresis – SDS-) introduction: a. Abou

write introduction and discussion (Electrophoresis – SDS-)
introduction: a. About 1-2 paragraphs b. THE SPECIFIC AIM OF THE EXPERIMENT c. Background information on the experiment d. What to expect in the experiment? e. an introduction that you would give a reader who does not have any background information on what the experiment will entail. f. You may use information from the PDF and other sources
discussion :
Summarize the main objective and the main results of this experiment within less than five lines paragraph. Why there is difference in the relative mobility of the studied proteins? Compare the bands of your samples, e.g. number of bands, size, relative mobility, and density of bands of each sample? Give an explanation for the differences and similarity of bands? What is the function of the chemicals that you used in gel preparation? Why you mixed SDS , Glycerol, buffer, and % Bromophenol with the sample buffer? Is that suitable to use tap water instead of Deionized water in this experiment? What is the advantage or disadvantage of this method that you used in this experiment? What is the main difference between SDS-PAGE, and native PAGE, and gPAGE

1.      Why did evolution favor glucose as the blood sugar, rather than other s

1.      Why did evolution favor glucose as the blood sugar, rather than other sugars (e.g., galactose, fructose, or sucrose)?
2.      Explain the metabolic origin of acidosis in chronic obstructive pulmonary disease.
3.      Explain the different roles of the pentose phosphate pathway in erythrocytes and nucleated cells.
4.      Explain the origin of drug-induced hemolytic anemia in persons with G6PD deficiency.
5.      Explain why some genetic mutations in α-globin or β-globin result in a pathologic phenotype, whereas most remain silent or benign. Describe the mutations that are the most difficult to detect.

Reflection Questions Week 5 1. Identify the major types of cellular RNA and the

Reflection Questions Week 5
1. Identify the major types of cellular RNA and the function of each.
2. Describe the major steps in the transcription of an RNA molecule.
3. Explain the function of the different RNA polymerase enzymes.
4. Explain how small RNAs regulate mRNA expression
5. Review the pathogenesis of systemic lupus erythematosus (SLE), an autoimmune disease in which antibodies to ribonucleoprotein particles are implicated in the development of chronic inflammation. The clinical presentation of SLE includes swelling of joints, fever > 100°F, hair loss, nose or mouth sores, and skin rash following sun exposure. Explain how each of these symptoms may result from SLE pathogenesis.

1. Identify the major types of cellular RNA and the function of each. 2. Describ

1. Identify the major types of cellular RNA and the function of each.
2. Describe the major steps in the transcription of an RNA molecule.
3. Explain the function of the different RNA polymerase enzymes.
4. Explain how small RNAs regulate mRNA expression
5. Review the pathogenesis of systemic lupus erythematosus (SLE), an autoimmune disease in which antibodies to ribonucleoprotein particles are implicated in the development of chronic inflammation. The clinical presentation of SLE includes swelling of joints, fever > 100°F, hair loss, nose or mouth sores, and skin rash following sun exposure. Explain how each of these symptoms may result from SLE pathogenesis.

1 Speculate on the mechanisms by which an adult with sickle cell disease would b

1 Speculate on the mechanisms by which an adult with sickle cell disease would benefit from a fetal hemoglobin (HbF) level of 20%.
2 Many hemoglobin-based oxygen carriers (HBOC) and some hemoglobin variants have a decreased sensitivity to pH. Discuss the consequences of a reduced Bohr effect.