Written Report You will attach a discussion to your table. Your report is the m

Written Report
You
will attach a discussion to your table. Your report is the most
important part of the unknown identification process. In your
discussion, focus on the results you obtained and how you identified
your unknown based on a process of elimination. The discussion
portion of your report is to be typed, and double spaced.
You will staple the table to the written report (table first followed
by the report). Please do not include information about the
procedures you conducted. The steps for writing your discussion are:
List
all possible GI bacteria used in this laboratory exercise. (5
points)
Discuss
your Gram staining results; describe if your Gram staining results
allowed you to eliminate any of the possible bacteria as your
unknown. (10
points)
Note:
once you have eliminated bacteria based on Gram staining, you do not
need to continue to eliminate them.
Discuss
the results for your Durham tube fermentation tests. Describe how
you used the three fermentation tubes to rule out certain bacteria.
You need to describe phenol red glucose, lactose and mannitol
results as one series of tests; do not describe each sugar
fermentation test separately. Be sure to clearly list which
bacteria are included and/or excluded based on the series of Durham
tube fermentation results and give clear reasons why.
(20 points)
Note:
once you have eliminated bacteria based on Durham tube fermentation,
you do not need to continue to eliminate them.
Discuss
several tests that clearly allowed you to identify your unknown
(these are known as definitive tests). For this part of your
report, you cannot include Gram staining and phenol red glucose,
lactose and mannitol since you have already discussed them in parts
b and c. Give reasons why certain organisms are excluded, and how
you reached your specific decision on the identity of your unknown.
You should describe how you excluded bacteria that remained as your
possible unknown after Gram staining and Durham tube fermentation.
Do not include a description of all
the
tests you performed. Include only the most relevant tests and
explain how they allowed you to eliminate the remaining
bacteria that were not previously excluded
and determine the identity your unknown. (20
points)
State
your unknown number and the identity of your unknown. (10
points)
Conclude
your report with a summary statement of how you were able to
identify your unknown. (5
points)