1) Title of Report: The title should be approximately 10 words (or can be less)

1) Title of Report: The title should be approximately 10 words (or can be less) and should encapsulate the main focus of the lab exercise. It is not an introductory statement – it should not be in the form of a complete sentence or a paragraph! Does not have to be a separate page.
2) Introduction: You should present the scientific purpose(s) and / or objective(s) of the experiment or exercise being performed. It should also provide the reader with some background information about the subject. In some cases, you may want to provide some background information about the organism(s) being worked with and / or some theory behind the techniques being used. This section provides “context” for the exercise.
3) Materials: You should provide a short list of what you consider to be the most important items used for the exercise. This would include the specific microorganisms used for the experiment, properly written out i.e. genus and species names. Also, if any of the materials are different than those cited in the lab manual, these should be included here. This does not need to be an exact replica of the materials list in the lab manual.
4) Methods: You should describe preferably in your own words what it is that you did in the lab. As above, if the procedure(s) differed from what was cited in the lab manual then you should report those differences here. Again, this should not an exact replica of the procedure in the lab manual. If you report that we did something that we did not perform, it will be noticed and might very well affect your grade for the report.
5) Results: You should present the outcomes of your experiments and observations without discussing their meaning or “implications”. Results can be presented as descriptions of observations, drawings with labels (a brief description is strongly recommended especially for those of you who do not draw well), tables and / or graphs. Tables and graphs should be properly labeled to understand what is being presented. Photographs alone are NOT acceptable results (this is the case regardless of whether they are your own or from Google.)
6) Conclusion/Discussion: Do NOT represent your results in this section; however, you will have to refer back to them as you “interpret” and discuss the significance of your results. For example, in performing the particular experiment or exercise, did you achieve your objective(s)? If not, why not? Can you provide suggestions for improvement? Even when the experiment /exercise did work, it is often appropriate to discuss reasons why the experiment might not work on other occasions. In most cases, it would be appropriate in this section to relate your results to existing relevant theory and / or knowledge. I will assign questions from the lab manual, which you need to answer in this section, to help with this aspect of the lab report. This will be provided to you shortly – see following pages.
7) References: Minimally, this should include the lab “manual”, i.e. lab materials being provided to you; however, in many cases, you may need to cite the textbook as well. If you are using websites and other resources, these should be cited as well. For websites, the address should be provided and the date that the website was accessed.
LAB REPORT 7- Selected questions from Exercises 5-1 & 5-2 & 5-18 & 5-19 ( aka intracellular enzymes & multiple-test media)
Bacteria to be used in exercises regarding the phenol red glucose media and the OF media include: E. coli, Pseudomonas aeruginosa, Proteus vulgaris
Bacteria to be used in the exercises regarding the SIM and TSIA media include: E. coli, Enterobacter aerogenes, S. aureus, Proteus vulgaris, Pseudomonas aeruginosa, Serratia marcescens (the specific bacteria depend on the media.)
Q1. All enterics are facultative anaerobes. What color results would you expect for organisms in O-F glucose media inoculated with an enteric. Remember to describe both sealed and unsealed tubes. How would an obligate aerobe appear in both the sealed and unsealed tubes? What would happen if lactose were used instead of glucose? Would you expect the results for E. coli and P. vulgaris to be identical? Explain based on your knowledge from how these two bacteria grow on MacConkey media.
Q2. a)With respect to the phenol red glucose media, what is the Durham tube and what is its purpose?
b)What is the purpose of the mineral oil for the OF media?
Q3. List two advantages of a multi-test system such as triple sugar iron agar or SIM.