I‌‍‍‍‌‍‍‌‌‍‍‍‌‍‍‍‍‌‍‍nvestigate the effects of methyl donor supplements (folic a

I‌‍‍‍‌‍‍‌‌‍‍‍‌‍‍‍‍‌‍‍nvestigate the effects of methyl donor supplements (folic acid) during pregnancy on offspring neural tube defects of obese sheep.( using sheep as experimental model) When will the intervention be studied?- Before and/or during gestation? • What parameters will be evaluated? ( at least 2): Skeletal muscle, kidneys, heart, liver, brain, gut, placenta, etc. ? Physiological parameters, PCR, Western blot, mitochondria, histology, Flow Cytometry. o When the measurements will be performed? ? During fetal/placental development, childhood or adulthood Mention maternal obesity effects on: 1‌‍‍‍‌‍‍‌‌‍‍‍‌‍‍‍‍‌‍‍. Placental development, structure and function 2. Offspring (neonate, childhood or adulthood) increased the risk of developing cardio-metabolic diseases • What intervention is necessary? Explore the importance of Omega-3 and folic acid supplementation. Be specific which organ/tissue it affects and why this organ/tissue is important, what is the action of folic acid? * What will this intervention increase/decrease in the body…(the metabolism activation of which path..? * look at histology, cell structure difference) the plan of the research proposal would be attached later in my ‌‍‍‍‌‍‍‌‌‍‍‍‌‍‍‍‍‌‍‍account